GAPDH antibody (AA 1-335)

Catalog No. ABIN3020538

The Rabbit Polyclonal anti-GAPDH antibody has been validated for WB, IHC, IF and IP. It is suitable to detect GAPDH in samples from Human. There are 13+ publications available.

Quick Overview for GAPDH antibody (AA 1-335) (ABIN3020538)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-GAPDH Antibody

Binding Specificity: AA 1-335

Sequence: MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Loading control Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH (NP_002037.2).

Isotype: IgG

Application Details

Application Notes: WB,1:1000 - 1:10000,IHC,1:50 - 1:100,IF,1:20 - 1:50,IP,1:20 - 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-GAPDH antibody (ABIN3020538)

Li, Luo, Wang, Liu, Chen, Zhao, Tan, Wang, Qin, Li, Xu, Yang: "The REN4 rheostat dynamically coordinates the apical and lateral domains of Arabidopsis pollen tubes." in: Nature communications, Vol. 9, Issue 1, pp. 2573, (2018) (PubMed).

Guo, Song, Zhao, Qi, Zhao: "Overexpression of Annexin A2 Receptor Inhibits Neovascularization via the Promotion of Krüppel-Like Transcription Factor 2." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 46, Issue 4, pp. 1617-1627, (2018) (PubMed).

Xue, Zhang, Wang, Zhang, Xiang, Wang, Wang, Li, Zhang, Zou, Wang, Wu, Lu, Chen, Ding, Li, Xu: "Benzoxazinone-containing 3,5-dimethylisoxazole derivatives as BET bromodomain inhibitors for treatment of castration-resistant prostate cancer." in: European journal of medicinal chemistry, Vol. 152, pp. 542-559, (2018) (PubMed).

Zhou, Liu, Wang, Huang, Li, Zhu, Wang, Ma, Li, Wei, Li, Li, Li, Xiao, Zhao: "Paeoniflorin attenuates ANIT-induced cholestasis by inhibiting apoptosis in vivo via mitochondria-dependent pathway." in: Biomedicine & pharmacotherapy, Vol. 89, pp. 696-704, (2018) (PubMed).

Cui, Zhang, Liu, Yan, Huang, Yang, Zhu, Sang: "Inhibition of TBK1 reduces choroidal neovascularization in vitro and in vivo." in: Biochemical and biophysical research communications, Vol. 503, Issue 1, pp. 202-208, (2018) (PubMed).

"Retracted: 'Anti-fibrotic actions of Ghrelin by inhibition of the NADPH oxidase-ROS signaling pathway' by Qian Wang, Xin Sui, Rui Chen, Peiyong Ma, Tao Ding, Dianjun Sui, and Ping Yang." in: Clinical and experimental pharmacology & physiology, Vol. 45, Issue 8, pp. 885, (2018) (PubMed).

Ding, Wen: "Dandelion root extract protects NCM460 colonic cells and relieves experimental mouse colitis." in: Journal of natural medicines, Vol. 72, Issue 4, pp. 857-866, (2018) (PubMed).

Luo, Cai, Luo, Tang, Meng: "Silencing activating transcription factor 2 promotes the anticancer activity of sorafenib in hepatocellular carcinoma cells." in: Molecular medicine reports, Vol. 17, Issue 6, pp. 8053-8060, (2018) (PubMed).

Zhu, Ye, Ashraf, Li, Chen, Song, Cao: "Transcriptional regulation of miR-15b by c-Rel and CREB in Japanese encephalitis virus infection." in: Scientific reports, Vol. 6, pp. 22581, (2017) (PubMed).

Wu, Jiang, Gong, Dou, Song, Song, Tian: "Vitamin E Reversed Apoptosis of Cardiomyocytes Induced by Exposure to High Dose Formaldehyde During Mice Pregnancy." in: International heart journal, Vol. 58, Issue 5, pp. 769-777, (2017) (PubMed).

Ma, Zhang, Wang, Li, Wei, Zhao, Jin, Li, Jin: "Tanshinones suppress AURKA through up-regulation of miR-32 expression in non-small cell lung cancer." in: Oncotarget, Vol. 6, Issue 24, pp. 20111-20, (2016) (PubMed).

Wang, Tian, Yong, Zhu, Lin, Jiang, Li, Huang, Lin: "Immunotoxicity assessment of CdSe/ZnS quantum dots in macrophages, lymphocytes and BALB/c mice." in: Journal of nanobiotechnology, Vol. 14, pp. 10, (2016) (PubMed).

Wu, Luo, Jiang, Wang, Wang, Wang, Fu, Li, Shi: "Marine bromophenol bis (2,3-dibromo-4,5-dihydroxy-phenyl)-methane inhibits the proliferation, migration, and invasion of hepatocellular carcinoma cells via modulating β1-integrin/FAK signaling." in: Marine drugs, Vol. 13, Issue 2, pp. 1010-25, (2016) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.,G3PD,GAPD,HEL-S-162eP,GAPDH,Cancer,Signal Transduction,Endocrine & Metabolism,Carbohydrate metabolism,Neuroscience,Neurodegenerative Diseases,Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer's Disease,GAPDH

Molecular Weight: 31 kDa/36 kDa

Gene ID: 2597

UniProt: P04406